Journal: Marine Drugs

Article Title: Physicochemical and Biocompatibility Properties of Type I Collagen from the Skin of Nile Tilapia ( Oreochromis niloticus ) for Biomedical Applications

doi: 10.3390/md17030137

Figure Lengend Snippet: The effect of ASC, PSC, and porcine collagen (PC) on cell proliferation. ( A ): MC3T3E1, ( B ): L929, ( C ): HUVEC. Values with * show significant differences ( p < 0.05) between groups, as determined by one-way ANOVA.

Article Snippet: MC3T3E1 and L929 cells were obtained from a cell bank (Procell Life Science & Technology Co., Ltd., Wuhan, China) and HUVEC were received from an institute of zoology (Chinese academy of sciences, Beijing, China).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr 2+ and Zn 2+ Ions Related to Their Influence on Biological Activities

doi: 10.3390/cimb44120425

Figure Lengend Snippet: Influence of Zn 2+ and Sr 2+ ions co-doped silicate-substituted hydroxyapatite nanoparticles on the viability of ( A ) TC28A2 and ( B ) 7F2 cell lines.

Article Snippet: The TC28A2 human chondrocyte cell line and 7F2 mouse osteoblast cell line were maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with L-glutamine (Biowest, Nuaillé, France) and supplemented with 10% Fetal Bovine Serum (FBS) South America Heat Inactivated (Biowest), 200 U/mL penicillin and 200 μg/mL streptomycin.

Techniques:

Journal: PeerJ

Article Title: miR-452-3p inhibited osteoblast differentiation by targeting Smad4

doi: 10.7717/peerj.12228

Figure Lengend Snippet: (A) The cell viability of MC3T3-E1 after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the osteoblast marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The mouse osteoblast cell line (MC3T3-E1) was purchased from ScienCell (Carlsbad, CA, USA) and cultured in alpha-minimum essential medium (α-MEM; SH30265.01B; Hyclone, South Logan, UT, USA) containing 1% penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA), 10% foetal bovine serum (FBS).

Techniques: CCK-8 Assay, Staining, Marker, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Expressing