Journal: Current Issues in Molecular Biology

Article Title: The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr 2+ and Zn 2+ Ions Related to Their Influence on Biological Activities

doi: 10.3390/cimb44120425

Figure Lengend Snippet: Influence of Zn 2+ and Sr 2+ ions co-doped silicate-substituted hydroxyapatite nanoparticles on the viability of ( A ) TC28A2 and ( B ) 7F2 cell lines.

Article Snippet: The TC28A2 human chondrocyte cell line and 7F2 mouse osteoblast cell line were maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with L-glutamine (Biowest, Nuaillé, France) and supplemented with 10% Fetal Bovine Serum (FBS) South America Heat Inactivated (Biowest), 200 U/mL penicillin and 200 μg/mL streptomycin.

Techniques:

Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: CBG, CBC, CBN, and CBDV effect on HuOB cell line viability. HuOB were treated for 72 h and then MTT assay was used to determine cell viability. Three separate experiments are shown as mean ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus VHC.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: MTT Assay

Journal: Biofactors (Oxford, England)

Article Title: Anticancer effect of minor phytocannabinoids in preclinical models of multiple myeloma

doi: 10.1002/biof.2078

Figure Lengend Snippet: MM cell invading the ECM covered membrane toward HuOB cells, after treatment with CBG, CBC, CBN, and CBDV. (A) Representative image of calcein stained RPMI cells invading the ECM coated membrane. Three random fields were observed under a fluorescence microscope. Magnification 10×. (B) The number of invading cells represent the mean ± SD of three separated experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001 versus UNTREATED.

Article Snippet: Human osteoblast cell line CI‐huOB (HuOB) was purchased from InSCREENex GmbH (Braunschweig, Germany) and cultured in DMEM glucose high medium (EuroClone, Milan, Italy) supplemented with 100 IU/mL penicillin, 100 mg streptomycin, 10% FBS, 1 mM sodium pyruvate, and 2 mM L‐glutamine.

Techniques: Membrane, Staining, Fluorescence, Microscopy